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I spent this morning doing slot-blot filter binding assays, which are an experiment in which you measure protein-nucleic acid (DNA or RNA) interactions using a double membrane sandwich with a nitrocellulose membrane on top that traps proteins (and bound nucleic acids) and a nylon membrane below that binds any free nucleic acids. So I planned to make a video showing you how I set it up and run it - and I did - but I also found I had some stuff to say about experimenting in general so I put that stuff first and then the behind-the-scenes look at the blot-a-lot-a-lotting If you want way more details…. much more about slot blotting: http://bit.ly/bindingaffinity more about Kd: http://bit.ly/bindingaffinity more about phosphor storage screens: http://bit.ly/phosphorimaging blog form: https://bit.ly/slotblotalot
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After spending some quality time with the family we are back and trying out some new games at four winds casino.
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Maybe I should also do small movie reviews on my channel. Wrapping up my movie-laden Saturday is the 1967 film adaptation of Ian Fleming’s novel before Daniel Craig popularized it less than 40 years later. Overall, it’s got a good story, a great soundtrack from the late great Burt Bacharach, and quite an interesting vibe despite it not being in the official James Bond canon. And yes, there were some parts that I found funny. In case you were wondering, I saw the film on Tubi. 8.5/10
